84-2045-05　［取扱停止］Neutrophil elastase, NT (ELANE, ELA2, Neutrophil elastase, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase) (PE) 200ul　038921-PE

特徴

• Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin.
• Humans have six elastase genes which encode the structurally similar proteins.
• The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils.
• The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix.
• This protein degrades the outer membrane protein A (OmpA) of E.
• coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia.
• Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN).
• This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter.
• All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
• Applications:Suitable for use in FLISA and Western Blot.
• Other applications have not been tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.