BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus.
It may play a role in the postsynaptic responses of hippocampal neuron.
Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), also known as cardio-facio-cutaneous syndrome.
CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation.
Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy.
Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition.
Typical facial features are similar to Noonan syndrome.
They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices.
The inheritance of CFC syndrome is autosomal dominant.
Applications:Suitable for use in FLISA and Dot Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000 Dot Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human BRAF when phosphorylated at Thr439.
Isotype：IgG
Calc Applications Abbrev：DB FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr439 of human BRAF (KLH).
