LDLRAP1 is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain.
The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor.
This adapter protein is required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts).
LDLRAP1 may be required for LDL binding and internalization but not for receptor clustering in coated pits.
This protein may facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits, and may also be involved in the internalization of other LDLR family members.
Mutations in the LDLRAP1 gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:50-1:100Immunohistochemistry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human LDLRAP1.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human LDLRAP1 (KLH).
