84-1985-38　［取扱停止］ZP4, CT (ZP4, ZPB, Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4) (Azide free) (HRP) 200ul　044405-HRP

特徴

• The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo.
• It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development.
• The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain.
• It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix.
• However, the requirement for furin cleavage in this process remains controversial based on mouse studies.
• Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1.
• However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500Immunohistochemistry: 1:10-50
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.