The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction.
Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments.
The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction.
The troponin complex has protein subunits C, I, and T.
Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction.
Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity.
There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle.
This gene encodes fast skeletal troponin T protein; also known as troponin T type 3.
Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms.
A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs.
Additional splice variants have been described but their biological validity has not been established.
Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：TNNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the C-terminal region of human TNNT3.
