TLR5, a Type I membrane protein belonging to the Toll-like receptor family, participates in the innate immune response to microbial agents.
It also plays a role in mediating detection of bacterial flagellins.
TLR5 acts via MyD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
This protein binds to TIRAP and MyD88 via their respective TIR domains TLR5 is highly expressed in liver, and is detected in lung and at very low levels in most other tissues.
The TLR5 gene lies in a locus that is associated with susceptibility to Salmonella.
Inbred strains of mice can be classified into 3 categories according to their resistance to infection with S.typhimurium: susceptible (BALB/c, C57BL/6, C3H/He), intermediate (DBA/2, C75L) and resistant (A, CBA).
The strain MOLF/Ei is highly susceptible to the infection, has an unique TLR5 haplotype and a lower expression of TRL5.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human TLR5. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the N-terminal region of mouse TLR5 (KLH).
