Component of the THO subcomplex of the TREX complex.
The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA.
It is recruited to spliced mRNAs by a transcription-independent mechanism.
Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export.
The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1.
UAP56 functions as a bridge between THOC4 and the THO complex.
The TREX complex is essential for the export of Kaposi's sarcoma-associated Herpes virus (KSHV) intronless mRNAs and infectious virus production.
The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4.
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human, mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：THOC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the Central region of human THOC6.
