The murine gene Suv39h2 encodes an H3 histone methyltransferase (HMTase) 59% identical in sequence to mouse Suv39h1.
During embryogenesis, both proteins overlap in tissue expression, yet Suv39h2 transcripts are restricted to the testes in adult animals.
Immunolocalization of the Suv39h2 protein during spermatogenesis indicates enrichment at the heterochromatin from the leptotene to the round spermatid stage.
Moreover, Suv39h2 specifically accumulates with chromatin of the sex chromosomes, which undergo transcriptional silencing during the first meiotic prophase.
Suv39h2 HMTase may also organize meiotic heterochromatin with the potential for epigenetic imprint to the male germline.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human SUV39H2. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 360-390 in the C-terminal region of human SUV39H2, UniProt Accession #Q9H5I1. Species sequence homology: monkey
