Stk38,also known as NUCLEAR DBF2-RELATED PROTEIN, contains 1 protein kinase domain that interacts with mob1 and mob2.The homodimeric s100b binds to two molecules of stk38.
It is ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Stk38 activated by binding of s100b which releases autoinhibitory n-lobe interactions, enabling atp to bind and the autophosphorylation of ser-281.
thr-444 then undergoes calcium- dependent phosphorylation by an upstream kinase.
Interactions between phosphorylated thr-444 and the n-lobe promote additional structural changes that complete the activation of the kinase.
Autoinhibition is also released by the binding of mob1/mobkl1a and mob2/hcca2 to the n-terminal of stk38.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:250-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human STK38.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human STK38 (KLH).
