84-1966-66　［取扱停止］SPNXB, ID (SPANXB1, SPANXB, Sperm protein associated with the nucleus on the X chromosome B/F, Cancer/testis antigen 11.2, Nuclear-associated protein SPAN-Xb, Nuclear-associated protein SPAN-Xf, SPANX family member B/F) (Azide free) (HRP) 200ul　042247-HRP

特徴

• Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa.
• This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X.
• The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments.
• This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B1 and appears to be a duplication of that locus.
• The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members.
• Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa.
• This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis.
• Applications:Suitable for use in Western Blot, ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.