The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization.
Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens.
The protein expressed by this gene is recognized by anti-sperm agglutinating antibodies from an infertile woman.
Furthermore, immunization of female rats with the recombinant human protein reduced fertility.
This protein localizes to the plasma membrane of germ cells in the testis and to the post-acrosomal plasma membrane of mature spermatozoa.
Recombinant polypeptide binds GTP and exhibits GTPase activity.
Thus, this protein may regulate GTP signal transduction pathways involved in spermatogenesis and fertilization.
Two transcript variants of this gene encode the same protein.
[provided by RefSeq].
Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：SPAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the N-terminal region of human SPAG1.
