Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD).
It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription.
It may act as a tumor suppressor.
Applications:Suitable for use in ELISA, Dot Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Dot Blot: 1:500Immunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human SMAD4 when phosphorylated at Thr277.
Isotype：IgG
Calc Applications Abbrev：DB E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding Thr277 of human SMAD4 (KLH).
