SIGLEC10 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells.
It preferentially binds to alpha2,3- or 2,6-linked sialic acid.
The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.
SIGLEC10 interacts with PTPN6/SHP-1 upon phosphorylation.
The protein is expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation).
Isoform 5 is found to be the most abundant isoform, found in lymph node, lung, ovary and appendix.
Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord.
Isoform 2 is also found in brain.
Isoform 4 is specifically found in natural killer cells.
SIGLEC10 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM).
This motif is involved in downmodulation of cellular responses.
The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1.
Phosphorylation of Tyr-667 is involved in binding to PTPN6.
The SIGLEC10 gene belongs to the immunoglobulin superfamily.
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：SIGLEC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human SIGLEC10.
