Phosphoribosylpyrophosphate synthetase (PRPS; EC 2.7.6.1) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine, pyrimidine, and pyridine biosynthesis.
By PCR of human lymphoblast mRNA using primers based on the cDNA sequence of rat PRS I (Prps1), Roessler et al.
(1990) isolated a partial human PRPS1 cDNA.
They used this partial cDNA to screen lymphoblast cDNA libraries and isolated additional cDNAs corresponding to the entire PRPS1 coding region.
The deduced PRPS1 protein has 318 amino acids.
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human, mouse
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：PRPS1/PRPS2/PRPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87~116 amino acids from the N-terminal region of human PRPS1/2/3.
