84-1946-76　［取扱停止］PEPCK2, NT (Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial), PEPCK-2, PEPCK, PCK2, Mitochondrial Phosphoenolpyruvate Carboxykinase 2, Phosphoenolpyruvate Carboxykinase [GTP] Mitochondrial, PEPCK-M, PEP Carboxykinase, Phosphoenolpyruvate Carboxylase) (Azide free) (HRP) 200ul　P3240-04N-HRP

特徴

• This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family.
• The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP.
• A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver.
• The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form.
• Alternatively spliced transcript variants have been described.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.