PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases.
PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop.
PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate.
Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis.
Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
Applications:Suitable for use in ELISA and Dot Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000 Dot Blot: 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human PDPK1 when phosphorylated at Ser393.
Isotype：IgG
Calc Applications Abbrev：DB E
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser396 of human PDPK1 (KLH).
