84-1944-41　［取扱停止］PAK3 (p21 Activated Kinase 3, p21-activated Kinase 3, p21(CDKN1A)-activated Kinase 3, PAK-3, PAK3beta, Beta PAK, Beta-PAK, bPAK, CDKN1A, hPAK3, Mental Retardation X-linked 30, MRX30, MRX47, Oligophrenin 3, OPHN3, Serine/threonine Protein Kinase PAK 3) (Azide free) (HRP) 200ul　P2488-20D-HRP

特徴

• PAK3, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets.
• PAK3 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1.
• It shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK.
• This protein is highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.
• PAK3 is autophosphorylated when activated by CDC42/p21.
• Defects in PAK3 are the cause of non-specific X-linked nonsyndromic mental retardation type 30 (MRX30).
• The protein structure contains 1 CRIB domain.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-1:100Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.