84-1944-40　［取扱停止］PAK2, NT (p21 Activated Kinase 2, p21-activated Kinase 2, p21(CDKN1A)-activated Kinase 2, PAK-2, Gamma PAK, hPAK65, p58, p65-PAK, PAK65, PAKgamma, S6/H4 Kinase, Serine/threonine Protein Kinase PAK 2) (Azide free) (HRP) 200ul　P2488-12R-HRP

特徴

• PAK2, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets.
• It phosphorylates ribosomal protein S6, histone H4 and myelin basic protein.
• PAK2 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1.
• Expression is ubiquitous, with higher levels seen in skeletal muscle, ovary, thymus and spleen.
• PAK2 is autophosphorylated when activated by CDC42/p21.
• The protein structure contains 1 CRIB domain.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.