84-1944-37　［取扱停止］PAK1 (T423) (Serine/Threonine-protein Kinase PAK 1, p21-activated Kinase 1, PAK-1, p65-PAK, Alpha-PAK) (Azide free) (HRP) 200ul　P2488-01R-HRP

特徴

• PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets.
• It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway.
• Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
• The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1.
• PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins.
• It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1.
• The protein is autophosphorylated when activated by CDC42/p21.
• Structurally, the PAK1 contains 1 CRIB domain.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-1:100Immunohistochemistry: 1:10-1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.