Three isoforms of nitric oxide synthase (NOS) have been identified.
All are homodimers with subunits of 130-160 kDa.
All have binding sites for NADPH, FAD, and FMN near the carboxyl terminus (the reductase domain), and binding sites for tetrahydrobiopterin (BH4) and heme near the amino terminus (the oxygenase domain).
The reductase and oxygenase domains are linked by a calmodulin (CaM) binding site.
Occupation of this site facilitates electron transfer from the cofactors in the reductase domain to heme during nitric oxide production.
NOS catalyzes the conversion of arginine to citrulline and nitric oxide (NO).
Neuronal nitric oxide synthase (nNOS, bNOS, cNOS, Type I) is associated with the post-synaptic density protein (PSD-95) in the neuronal membrane.
In response to increased intracellular Ca2+, nNOS interacts with CaM.
The Ca2+ CaM complex, in combination with BH4, binds to nNOS and induces its translocation from the plasma membrane to the cytoplasm.
The dephosphorylation of nNOS by calcineurin initiates the production NO.
NO activates guanylyl cyclase (GC) and activates the various cGMP regulated signaling pathways.
nNOS is in activated by phosphorylation by protein kinase A (PKA) or protein kinase C (PKC).
Applications:Suitable for use in Dot Blot, ELISA
Recommended Dilution:ELISA: 1:1,000Dot blot 1:500
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：DB E
Calc Crossreactivity：Hu
Immunogen：Phospho-nNOS-S1417 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1403~1432 amino acids surrounding S1417 of human nNOS.
