84-1932-13　［取扱停止］MBNL1, CT (MBNL1, EXP, KIAA0428, MBNL, Muscleblind-like protein 1, Triplet-expansion RNA-binding protein) (Azide free) (HRP) 200ul　038246-HRP

特徴

• Mediates pre-mRNA alternative splicing regulation.
• Acts either as activator or repressor of splicing on specific pre-mRNA targets.
• Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle.
• Antagonizes the alternative splicing activity pattern of CELF proteins.
• Regulates the TNNT2 exon 5 skipping through competition with U2AF2.
• Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA.
• Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly.
• Binds to the 5'-YGCU(U/G)Y-3'consensus sequence.
• Binds to the IR RNA.
• Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.