84-1929-99　［取扱停止］LY6E, ID (LY6E, 9804, RIGE, SCA2, TSA1, Lymphocyte antigen 6E, Retinoic acid-induced gene E protein, Stem cell antigen 2, Thymic shared antigen 1) (Azide free) (HRP) 200ul　038014-HRP

特徴

• Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins.
• It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes.
• In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes.
• Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes.
• The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications have not been tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.