HSP90B1 is highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution.
HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress.
HSP90B1 is an endoplasmic reticulum HSP90 protein.
Other HSP90 proteins are found in cytosol.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes human HSP90B1. Species Crossreactivity: hamster and mouse
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hm Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 16-43 in the N-terminal region of human HSP90B1. Species Sequence Homology: bovine, chicken, monkey, porcine and rat