HRH3 is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the CNS and PNS.
The deduced 445aa HRH3 protein contains 7 predicted transmembrane domains.
HRH3 has significant sequence homology to members of the biogenic amine subfamily of GPCRs, including an aspartic acid residue in the predicted third transmembrane domain, a hallmark of the biogenic amine receptor subfamily; this residue is the putative binding site for the primary amine.
HRH3 shares 22% and 21.4% amino acid sequence homology with the H1 and H2 receptors, respectively.
Expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine.
Northern blot analysis of human tissues showed HRH3 expression only in the brain, with highest expression in the thalamus and caudate nucleus.
RT-PCR showed expression in human small intestine, testis, and prostate.
In situ hybridization of rat brain sections showed that HRH3 is abundantly expressed in brain.
HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus.
Strong expression was also seen in layers II, V, and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.
In addition, HRH3 expression was found in the locus ceruleus and in the histaminergic cell bodies in the tuberomammillary nuclei.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100-1:500Immunohistochemistry (FFPE): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human HRH3 at ~50kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a portion of amino acids within aa416-445 from the C-terminal region of human HRH3 conjugated to KLH (Q9Y5N1). Cellular Location: Cell Membrane
