84-1918-03　Hexokinase 1, NT (Hexokinase-1, Hexokinase Type I, HEX1, HK I, HKI, HK1, HXK1, Brain Form Hexokinase) (Azide free) (HRP) 200ul　H2035-25N-HRP

特徴

• Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway.
• The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria.
• Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency.
• Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific.
• Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms.
• HK1 encodes the ubiquitously expressed isoform.
• Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD).
• The porin binding domain mediates association with the mitochondrial membrane.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.