84-1907-06　［取扱停止］EPS15, CT (Epidermal Growth Factor Receptor Substrate 15, Protein Eps15, AF-1p protein, AF1P) (Azide free) (HRP) 200ul　E3376-04A-HRP

特徴

• EPS15 is involved in cell growth regulation, possibly via the regulation of mitogenic signals and control of cell proliferation.
• EPS15 also participates in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR.
• Potential interaction partners include AP2A2, STN2, EPN1, and CRK via its SH3-binding sites.
• EPS15 is ubiquitously expressed.
• Phosphorylation on Tyr-849 is involved in the internalization of EGFR.
• EPS15 is not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis.
• This protein is involved in a t(1;11)(p32;q23) chromosomal translocation in acute leukemias causing fusion to the trithorax (MLL or HRX) gene product which contains DNA-binding motifs resulting in a rogue activator protein.
• Structurally, EPS15 contains 2 EF-hand calcium-binding domains, 3 EH domains, and 2 ubiquitin-interacting motif (UIM) repeats.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.