84-1901-77 ［取扱停止］DENND1B, NT (DENND1B, C1orf218, FAM31B, DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B) (Azide free) (HRP) 200ul 034583-HRP
84-1901-77 ［取扱停止］DENND1B, NT (DENND1B, C1orf218, FAM31B, DENN domain-containing protein 1B, Connecdenn 2, Protein FAM31B) (Azide free) (HRP) 200ul 034583-HRP
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids.
Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024).
Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).
Applications:Suitable for use in Western Blot, Immunohistochemistry ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of human DENND1B.
