Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment.
These molecules are involved in the regulation of cell growth, differentiation and metabolism.
MCK10 is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen.
This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain.
Its autophosphorylation is achieved by all collagens so far tested (type I to type VI).
In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain.
In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain.
The gene is located on chromosome 6p21.3 in proximity to several HLA class I genes.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human DDR1. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 17-47 in the N-terminal region of human DDR1, Swiss/UniProt Accession: Q08345; Q63474; Q03146. Species Sequence Homology: mouse
