Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain.
It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane.
The complex consists of 13 mitochondrial- and nuclear-encoded subunits.
The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities.
The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex.
This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme.
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：COX5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-49 amino acids from the Central region of human COX5B.
