Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin.
Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B.
Unlike other elastases, elastase 3A has little elastolytic activity.
Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine.
Elastase 3A preferentially cleaves proteins after alanine residues.
Elastase 3A may also function in the intestinal transport and metabolism of cholesterol.
Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.
Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：CELA3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-186 amino acids from the Central region of human CELA3A.
