Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules.
This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines.
In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress.
However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon.
The majority of human individuals have the premature stop codon and produce a truncated non-functional protein.
The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis.
Several alternatively spliced transcript variants have been noted for this gene.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications have not tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human CASP12.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 165-193 in the central region of human CASP12, Swiss/UniProt Accession: Q6UXS9
