APOBEC1 is involved in the production of apolipoprotein B (apoB)-48 from apoB-100.
The gene spans 18 kb and contains five exons, all of which are translated.
Alternative splicing produces a variant transcript that lacks exon 2 and encodes a novel 36aa peptide.
The exon 2-skipped transcript accounts for approximately 50% of APOBEC1 mRNA in the adult small intestine and up to 90% of APOBEC1 mRNA in the developing gut.
Exon 2-skipping may thus be a quantitatively important mechanism for regulating the expression of this gene in the gastrointestinal tract.
Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Apobec1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the N-terminal region of human Apobec1 (KLH).
