YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins.
This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog.
It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue.
It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications have not tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human 14-3-3, gamma. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 63-92 in the N-terminal region of human 14-3-3, gamma. Species sequence homology: bovine, chicken, Xenopus, Zebrafish
