84-1872-47　［取扱停止］PKC nu (PRKD3, EPK2, PRKCN, Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu) (FITC) 200ul　040093-FITC

特徴

• Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol.
• PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways.
• PKC also serve as major receptors for phorbol esters, a class of tumor promoters.
• Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells.
• PKC nu is one of the PKC family members.
• This kinase can be activated rapidly by the agonists of G protein-coupled receptors.
• It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation.
• This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-500Immunohistochemistry: 1:50-100Immunofluorescence: 1:500
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.