Chromosome 11q23 translocations targeting mixed-lineage leukemia 1 (MLL) gene have been correlated with human acute leukemias, including lymphoid, myeloid, monoblastic and myelomonocytic varieties.
More than 3 dozen genes that fuse to with MLL have been identified in various types of malignancies, including AF4, AF9, ENL, CBL, LARG, LPP, GMPS, and FBP17.
The 431 kDa MLL protein exhibits greatest abundancy in cerebral cortex, kidney, thyroid, and lymphoid tissues.
Inside the cell, MLL is part of a stable multiprotein supercomplex, the majority of which are components of transcription complexes, including TFIID.
The MLL multiprotein complex seems to remodel, acetylate, deacetylate, and methylate nucleosomes and/or free histones.
MLL contains a SET domain characteristic for histone H3 lysine-4-specific methyltransferases.
Oncogenic MLL fusion proteins appear to alter the methyltransferase activity of wild-type MLL.
Applications:Suitable for use in FLISA
Recommended Dilution:FLISA: 1:1,000
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：FLISA
Calc Crossreactivity：Hu
Immunogen：MLL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 3873~3902 amino acids from the C-terminal region of human MLL1.
