84-1867-72　［取扱停止］XBP1, ID (XBP1, TREB5, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5) (FITC) 200ul　043914-FITC

特徴

• XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box.
• This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter.
• It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.
• It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1).
• The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.
• Applications:Suitable for use in FLISA, Western Blot and Immunohistochemisty.
• Other potential application, though not tested is Direct Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: Formalin fixed and paraffin embedded tissues Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.