MUC1 is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein.
The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type.
It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals.
The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity.
Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas.
Applications:Suitable for use in Immunofluorescence, Dot Blot, FLISA
Recommended Dilution:FLISA: 1:1,000Dot blot 1:500Immunofluorescence: 1:10-50
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：DB FLISA IF
Calc Crossreactivity：Hu
Immunogen：Phospho-MUC1-Pt1224 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between amino acids 1210-1240 surrounding T1224 of human MUC1.
