SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation.
It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2.
Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunohistochemistry and FLISA.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human SMURF2. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 702-731 in the C-terminal region of human SMURF2. Species Sequence Homology: mouse, Xenopus.
