Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function.
In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes.
PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers.
PFKs of muscle and liver are homotetramers of the M and L subunits, respectively.
Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4.
PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate.
PFK catalyzes the key controlling step of glycolytic pathway.
PFK deficiency can present as mild to life-threatening episodic illness.
A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise.
The mean red cell PFK is elevated in persons with Down syndrome.
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human PFKL. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide selected from the C-terminal region of human PFKL (KLH).
