Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway.
The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria.
Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency.
Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific.
Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms.
HK1 encodes the ubiquitously expressed isoform.
Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD).
The porin binding domain mediates association with the mitochondrial membrane.
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human HK1.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 705-734 in the C-terminal region of human HK1, UniProt Accession #P19367.
