Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system.
Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins.
The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family.
EphB3, a member of the Tyr family of protein kinases, is a receptor for members of the ephrin-B family; it binds to both ephrin-B1 and -B2.
Expression of this Type I membrane protein is ubiquitous.
The protein contains putative domains for 2 fibronectin type III and 1 sterile alpha motif (SAM).
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A/G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human EphB3
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 29-59 in the N-terminal region of human EphB3. Species Sequence Homology: mouse
