Myb is a transcriptional activator; DNA-binding protein that recognizes the sequence 5'-YAAC[GT]G-3'.
It participates in the control of proliferation and differentiation of hematopoietic progenitor cells.
Myb is a transcription factor related to Myc, and is expressed predominantly in haematopoietic cells and immature gastroepithelial cells.
TRAF7 stimulates the sumoylation of Myb at Lys-523 and Lys-499, which are the same sites as those used for PIASy-induced sumoylation.
A correlation has been established between reduction in sumoylation of Myb and increase in transcriptional activation.
Negative influence on transactivation properties by the negative regulatory domain region of c-Myb depends on upon sumoylation.
Applications:Suitable for use in FLISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1000Immunohistochemistry (Paraffin): 1:50-1:100Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human MYB. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide PLLKKIKQEVESPTCA containing a predicted sumoylation site from the C-terminal region of human MYB (KLH).
