The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M.
Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis.
During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus.
The full-length cDNA encodes a 747aa protein with a putative ATP/GTP-binding site motif.
RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues.
Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney.
P100 is also correlated to cancer prognosis.
Applications:Suitable for use in FLISA, Western Blot, and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human P100.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：A mixture of 3 KLH-conjugated synthetic peptides selected from C-terminal region with phenylalanine at position 624, C-terminal region with serine at position 729, and center region of human NRG4 (KLH).
