84-1775-56　NES (S1409) (Nestin, Nbla00170) (FITC) 200ul　N2060-04L-FITC

特徴

• Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells.
• In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments.
• Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway.
• Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.
• Applications:Suitable for use in FLISA, Western Blot, Immunofluorescence and Immunohistochemisty.
• Other potential application, though not tested is Direct Flow Cytometry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: Formalin fixed, paraffin embedded tissues.
• Heat induced antigen retrieval with citrate buffer, pH 6.0 is recommended
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.