84-1765-70　［取扱停止］XRCC6, ID (G22P1, X-ray repair cross-complementing protein 6, 5'-deoxyribose-5-phosphate lyase Ku70, 70kD subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70kD subunit, CTC box-binding factor 75kD subunit, DNA repair protein XRCC6, Lupus Ku autoantigen protein p70, Thyroid-lupus autoantigen, X-ray repair complementing defective repair in Chinese hams ...　043936-Biotin

特徴

• Ku70 is a single stranded DNA-dependent ATP-dependent helicase.
• It has a role in chromosome translocation.
• The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner.
• It works in the 3'-5' direction.
• Binding to DNA may be mediated by p70.It is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
• The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold.
• The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry (Not Tested), ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-100Immunohistochemistry: 1:50-100
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.