84-1762-43　［取扱停止］ATP6V0A4, ID (ATP6V0A4, ATP6N1B, ATP6N2, V-type proton ATPase 116kD subunit a isoform 4, Vacuolar proton translocating ATPase 116kD subunit a isoform 4, Vacuolar proton translocating ATPase 116kD subunit a kidney isoform) (Biotin) 200ul　032293-Biotin

特徴

• This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells.
• V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation.
• V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain.
• The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits.
• The V1 domain contains the ATP catalytic site.
• The V0 domain consists of five different subunits: a, c, c', c'', and d.
• This gene is one of four genes in man and mouse that encode different isoforms of the a subunit.
• Alternatively spliced transcript variants encoding the same protein have been described.
• Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.
• Applications:Suitable for use in Western Blot and ELISA.
• Other potential application, though not tested is Direct Flow Cytometry.
• Other applications have not been tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.