84-1749-63　［取扱停止］GBL, CT (MLST8, GBL, LST8, Target of rapamycin complex subunit LST8, G protein beta subunit-like, Mammalian lethal with SEC13 protein 8) (Biotin) 200ul　035938-Biotin

特徴

• Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals.
• mTORC1 is activated in response to growth factors or amino-acids.
• Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system.
• Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1.
• Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis.
• mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E).
• mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.
• Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity.
• In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity.
• mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive.
• mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors.
• mTORC2 promotes the serum-induced formation of stress-fibers or F-actin.
• mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation.
• mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'.
• mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
• Applications:Suitable for use in Western Blot, ELISA
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-500
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.