Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends.
Prenylation was initially believed to be important only for membrane attachment.
However, another role for prenylation appears to be its importance in protein-protein interactions.
The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins.
Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif.
This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A.
The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain.
It may be a component of a prelamin A endoprotease complex.
The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina.
It shares limited sequence similarity with iron-only bacterial hydrogenases.
Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：NARF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the Central region of human NARF.
