The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction.
Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments.
The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction.
The troponin complex has protein subunits C, I, and T.
Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction.
Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity.
There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle.
This gene encodes fast skeletal troponin T protein; also known as troponin T type 3.
Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms.
A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs.
Additional splice variants have been described but their biological validity has not been established.
Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：TNNT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the C-terminal region of human TNNT3.
