Melanoma antigen preferentially expressed in tumors is a protein that in humans is encoded by the PRAME gene.1,2,3 which encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes.
It is not expressed in normal tissues, except testis.
This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE.
However, unlike these other CT antigens, this gene is also expressed in acute leukemias.
Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.3
Applications:Suitable for use in Western Blot, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：PRAMEF20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-431 amino acids from the C-terminal region of human PRAMEF20.
